Induction of apoptosis in human breast adenocarcinoma MCF-7 cells by pterocarnin A from the bark of Pterocarya stenoptera via the Fas-mediated pathway.

Abstract

Pterocarnin A, isolated from the bark of Pterocarya stenoptera (Juylandaceae), was investigated for its antiproliferative activity in human breast adenocarcinoma MCF-7 cells. To identify the anticancer mechanism of pterocarnin A, we assayed its effects on apoptosis, cell cycle distribution, and levels of p53, p21/WAF1, Fas/APO-1 receptor and Fas ligand. The results showed that pterocarnin A induced apoptosis of MCF-7 cells without mediation of p53 and p21/WAF1. We suggest that the Fas/Fas ligand apoptotic system is the main pathway of pterocarnin A-mediated apoptosis of MCF-7 cells. Our study reports here for the first time that the activity of the Fas/Fas ligand apoptotic system may participate in the antiproliferative activity of pterocarnin A in MCF-7 cells.

• Authors: Po-Lin Kuo, Ya-Ling Hsu, Shu-Chiao Sung, Wen-Chiu Ni, Ta-Chen Lin, Chun-Ching Lin
• Journal: Anti-cancer drugs (Anticancer Drugs) Vol. 18 Issue 5 Pg. 555-62 (Jun 2007) ISSN: 0959-4973 [Print] England
• PMID: 17414624 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Fas Ligand Protein
• Glycosides
• pterocarnin A
• Gallic Acid
• Caspase 8
• Oncogene Protein p21(ras)

Topics

• Adenocarcinoma (drug therapy, pathology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Breast Neoplasms (drug therapy, pathology)
• Caspase 8 (metabolism)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Enzyme-Linked Immunosorbent Assay
• Fas Ligand Protein (physiology)
• Gallic Acid (analogs & derivatives, pharmacology)
• Genes, p53 (genetics)
• Glycosides (pharmacology)
• Humans
• Juglandaceae (chemistry)
• Oncogene Protein p21(ras) (genetics)
• Plant Bark (chemistry)