Abstract | BACKGROUND & AIMS: METHODS: Using rhodamine-conjugated phalloidin staining, we evaluated whether tTG antibodies, both commercially available and cloned from patients with celiac disease, cause cytoskeletal changes in Caco-2, MCF7, and NIH 3T3 cells. We monitored cell levels of bromodeoxyuridine incorporation to determine whether tTG autoantibodies are able to induce NIH 3T3 fibroblasts and epithelial mucosal cells into S phase. RESULTS: Treatment with tTG antibodies caused a dose-dependent increase of membrane ruffling in Caco-2, MCF7, and NIH 3T3 cells. It also dose-dependently induced G(0)-synchronized NIH 3T3 fibroblasts into S phase but did not affect the rate of apoptosis. Similarly, tTG antibodies induced S-phase entry of epithelial cells in cultured intestinal biopsy specimens from patients with celiac disease. They did not affect biopsy specimens from patients without celiac disease. CONCLUSIONS:
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Authors | Maria V Barone, Ivana Caputo, Maria T Ribecco, Maria Maglio, Roberto Marzari, Daniele Sblattero, Riccardo Troncone, Salvatore Auricchio, Carla Esposito |
Journal | Gastroenterology
(Gastroenterology)
Vol. 132
Issue 4
Pg. 1245-53
(Apr 2007)
ISSN: 0016-5085 [Print] United States |
PMID | 17408665
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Autoantibodies
- Recombinant Proteins
- Protein Glutamine gamma Glutamyltransferase 2
- Transglutaminases
- GTP-Binding Proteins
- Bromodeoxyuridine
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Topics |
- Actins
(drug effects, metabolism)
- Animals
- Antibody Formation
(drug effects)
- Apoptosis
(immunology)
- Autoantibodies
(therapeutic use)
- Biopsy
- Bromodeoxyuridine
- Caco-2 Cells
(drug effects, immunology, pathology)
- Celiac Disease
(drug therapy, immunology, pathology)
- Cell Cycle
(drug effects, immunology)
- Cell Proliferation
(drug effects)
- Epithelial Cells
(drug effects, immunology, pathology)
- GTP-Binding Proteins
- Humans
- In Situ Nick-End Labeling
- Intestinal Mucosa
(drug effects, metabolism, pathology)
- Mice
- NIH 3T3 Cells
(drug effects, immunology, pathology)
- Protein Glutamine gamma Glutamyltransferase 2
- Recombinant Proteins
- Transglutaminases
(immunology)
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