Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains.

Abstract

Escherichia coli K12 strains producing L-phenylalanine were converted to L-tyrosine-producing strains using a novel genetic method for gene replacement. We deleted a region of the E. coli K12 chromosome including the pheA gene encoding chorismate mutase/prephenate dehydratase, its leader peptide (pheL), and its promoter using a new polymerase chain reaction-based method that does not leave a chromosomal scar. For high level expression of tyrA, encoding chorismate mutase/prephenate dehydrogenase, its native promoter was replaced with the strong trc promoter. The linked DeltapheLA and Ptrc-tyrA::Kan(R) genetic modifications were moved into L-phenylalanine producing strains by generalized transduction to convert L-phenylalanine-producing strains to L-tyrosine-producing strains. Moreover, introduction of a plasmid carrying genes responsible for sucrose degradation into these strains enabled L-tyrosine-production from sucrose.

• Authors: Monica M Olson, Lori J Templeton, Wonchul Suh, Philip Youderian, F Sima Sariaslani, Anthony A Gatenby, Tina K Van Dyk
• Journal: Applied microbiology and biotechnology (Appl Microbiol Biotechnol) Vol. 74 Issue 5 Pg. 1031-40 (Apr 2007) ISSN: 0175-7598 [Print] Germany
• PMID: 17216463 (Publication Type: Journal Article)

Chemical References

• Tyrosine
• Phenylalanine
• Sucrose
• Glucose

Topics

• Escherichia coli (genetics, metabolism)
• Glucose (metabolism)
• Phenylalanine (biosynthesis)
• Sucrose (metabolism)
• Tyrosine (biosynthesis)