Abstract | OBJECTIVE: DESIGN: SETTING: Research laboratory of a university hospital. SUBJECTS: Wild-type mice and PAF-receptor knockout mice. INTERVENTIONS: MEASUREMENTS AND MAIN RESULTS: Preinfusion with soybean oil resulted in increased leukocyte invasion, myeloperoxidase activity, and protein leakage and exaggerated release of tumor necrosis factor ( TNF)-alpha as well as macrophage inflammatory protein (MIP)-2 into the alveolar space after intratracheal lipopolysaccharide challenge. In contrast, preinfusion with fish oil reduced leukocyte invasion, myeloperoxidase activity, protein leakage, and TNF-alpha as well as MIP-2 generation. Corresponding profiles were found in plasma following intraperitoneal lipopolysaccharide application: Soybean oil increased but fish oil decreased the TNF-alpha and MIP-2 formation. When PAF-receptor-deficient mice were challenged with lipopolysaccharide, leukocyte invasion, lung tissue myeloperoxidase, cytokine generation, and alveolar protein leakage corresponded to those observed in wild-type animals. Fish oil and soybean oil lost their diverging effects on leukocyte transmigration, myeloperoxidase activity, leakage response, and cytokine generation in these knockout mice. Similarly, the differential impact of both lipid emulsions on these lipopolysaccharide-provoked changes was suppressed after pretreating animals with a PAF-receptor antagonist. CONCLUSIONS:
Fish oil- vs. soybean oil-based lipid infusions exert anti- vs. proinflammatory effects in murine models of acute inflammation. The PAF/PAF-receptor-linked signaling appears to be a prerequisite for this differential profile.
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Authors | Martina Barbara Schaefer, Juliane Ott, Andrea Mohr, Ming Hua Bi, Andrea Grosz, Norbert Weissmann, Satoshi Ishii, Friedrich Grimminger, Werner Seeger, Konstantin Mayer |
Journal | Critical care medicine
(Crit Care Med)
Vol. 35
Issue 2
Pg. 544-54
(Feb 2007)
ISSN: 0090-3493 [Print] United States |
PMID | 17205023
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokine CXCL2
- Chemokines
- Cxcl2 protein, mouse
- Fat Emulsions, Intravenous
- Fish Oils
- Platelet Membrane Glycoproteins
- Receptors, G-Protein-Coupled
- platelet activating factor receptor
- Soybean Oil
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Topics |
- Animals
- Chemokine CXCL2
- Chemokines
(physiology)
- Fat Emulsions, Intravenous
(pharmacology, therapeutic use)
- Fish Oils
(pharmacology, therapeutic use)
- Inflammation
(therapy)
- Leukocytes
(drug effects, immunology)
- Mice
- Mice, Knockout
- Platelet Membrane Glycoproteins
(physiology)
- Receptors, G-Protein-Coupled
(physiology)
- Respiratory Distress Syndrome
(therapy)
- Soybean Oil
(pharmacology, therapeutic use)
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