Abstract | BACKGROUND/AIMS: METHODS: Gene expression analysis was performed on liver of CBS-deficient mice using quantitative real-time PCR. RESULTS: We found that CBS-deficiency in liver mice significantly increases expression of genes induced by endoplasmic reticulum stress and genes that regulate the expression of enzymes required for cholesterol and fatty acid biosynthesis and uptake, notably the scavenger receptor class B type I (SR-BI), concomitant with overexpression of SR-BI at the protein level. Moreover, we also found increased mRNA levels of ABCG5, ABCG8, ABCG1 and ABCA1, which play important roles in reverse cholesterol transport, associated with an upregulation of liver X receptors and a downregulation of the peroxisome proliferators-activated receptor alpha. CONCLUSIONS:
|
Authors | Julien Hamelet, Karine Demuth, Jean-Louis Paul, Jean-Maurice Delabar, Nathalie Janel |
Journal | Journal of hepatology
(J Hepatol)
Vol. 46
Issue 1
Pg. 151-9
(Jan 2007)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 17030070
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- DNA Primers
- RNA, Messenger
- Receptors, Cytoplasmic and Nuclear
- Homocysteine
- Cholesterol
- Cystathionine beta-Synthase
|
Topics |
- Animals
- Base Sequence
- Cholesterol
(metabolism)
- Cystathionine beta-Synthase
(deficiency, genetics)
- DNA Primers
(genetics)
- Disease Models, Animal
- Endoplasmic Reticulum
(metabolism)
- Homocysteine
(blood)
- Homocystinuria
(complications, genetics, metabolism)
- Hyperhomocysteinemia
(enzymology, etiology, genetics)
- Lipid Metabolism
- Liver
(metabolism)
- Male
- Mice
- Mice, Knockout
- RNA, Messenger
(genetics, metabolism)
- Receptors, Cytoplasmic and Nuclear
(genetics)
|