Crinamine from Crinum asiaticum var. japonicum inhibits hypoxia inducible factor-1 activity but not activity of hypoxia inducible factor-2.

Abstract

In a search for natural product inhibitors of hypoxia inducible factor-1 (HIF-1) function, crinamine (1), a crinane type alkaloid, showed potent dose dependent inhibition (IC50 = 2.7 microM) of HIF-1alpha in a cell-based reporter gene assay. Crinamine (1) was isolated from the aerial parts of Crinum asiaticum var. japonicum together with lycorine (2), norgalanthamine (3) and epinorgalanthamine (4). The other components (2-4) showed no significant inhibition of HIF-1alpha induced transcriptional activity.

• Authors: Young Ho Kim, Eun Jung Park, Mi Hyun Park, Uranchimeg Badarch, Girma Moges Woldemichael, John Albert Beutler
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 29 Issue 10 Pg. 2140-2 (Oct 2006) ISSN: 0918-6158 [Print] Japan
• PMID: 17015967 (Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)

Chemical References

• Amaryllidaceae Alkaloids
• Basic Helix-Loop-Helix Transcription Factors
• Hypoxia-Inducible Factor 1
• Indolizines
• Transcription Factors
• endothelial PAS domain-containing protein 1
• crinamine
• Nitric Oxide Synthase Type II

Topics

• Amaryllidaceae Alkaloids (pharmacology)
• Basic Helix-Loop-Helix Transcription Factors
• Cell Line, Tumor
• Crinum (chemistry)
• Humans
• Hypoxia-Inducible Factor 1 (antagonists & inhibitors)
• Indolizines (pharmacology)
• Nitric Oxide Synthase Type II (physiology)
• Transcription Factors (antagonists & inhibitors)