Abstract |
A contributing factor to the pathology of Alzheimer's disease is the generation of reactive oxygen species, most probably a consequence of the beta-amyloid (Abeta) peptide coordinating copper ions. Experimental and theoretical results indicate that His13 and His14 are the two most firmly established ligands in the coordination sphere of Cu(II) bound to Abeta. Abeta1-42 is known to reduce Cu(II) to Cu(I). The Abeta-Cu(II) complex has been shown to catalytically generate H(2)O(2) from reducing agents and O(2). Cu(II) in the presence of Abeta has been reported to have a formal reduction potential of +0.72-0.77 V (vs. the standard hydrogen electrode). Quantum chemical calculations using the B3LYP hybrid density functional method with the 6-31G(d) basis set were performed to model the reduction of previously studied Cu(II) complexes representing the His13-His14 portion of Abeta (Raffa et al. in J. Biol. Inorg. Chem. 10:887-902, 2005). The effects of solvation were accommodated using the CPCM method. The most stable complex between Cu(I) and the model compound, 3-(5-imidazolyl)propionylhistamine (1) involves tricoordinated Cu(I) in a distorted-T geometry, with the Npi of both imidazoles as well as the oxygen of the backbone carbonyl bound to copper. This model would be the most likely representation of a Cu(I) binding site for a His-His peptide in aqueous solution. A variety of possible redox processes are discussed.
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Authors | Duilio F Raffa, Gail A Rickard, Arvi Rauk |
Journal | Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
(J Biol Inorg Chem)
Vol. 12
Issue 2
Pg. 147-64
(Feb 2007)
ISSN: 0949-8257 [Print] Germany |
PMID | 17013614
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amyloid beta-Peptides
- Dipeptides
- Ligands
- Peptide Fragments
- amyloid beta-protein (1-42)
- histidylhistidine
- Copper
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Topics |
- Alzheimer Disease
(metabolism)
- Amino Acid Sequence
- Amyloid beta-Peptides
(chemistry, metabolism)
- Binding Sites
- Computational Biology
- Copper
(chemistry, metabolism)
- Dipeptides
(chemistry, metabolism)
- Humans
- Ligands
- Models, Molecular
- Molecular Sequence Data
- Oxidation-Reduction
- Peptide Fragments
(chemistry, metabolism)
- Thermodynamics
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