Nitric oxide regulates Angiopoietin1/Tie2 expression after stroke.

Abstract

We tested whether the nitric oxide donor, (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) aminio] diazen-1-ium-1,2-diolate (DETA-NONOate), increases expression of Angiopoietin (Ang1)/Tie2, which may play a role in regulating angiogenesis and vascular integrity after stroke in rats. Wistar rats were subjected to middle cerebral artery occlusion and treated with or without DETA-NONOate. Stroke rats treated with DETA-NONOate show significantly increased Ang1, Tie2 and Occludin expression in the ischemic border compared with control stroke animals (p < 0.05). Consistent with in vivo data, DETA-NONOate promotes capillary tube formation in cultured brain endothelial cells. Neutralizing Ang1 antibody attenuates DETA-NONOate-induced capillary tube formation. The data suggest that the Ang1/Tie2 axis promotes DETA-NONOate-induced angiogenesis and stabilizes of angiogenic vessels after stroke.

• Authors: Alex Zacharek, Jieli Chen, Chunling Zhang, Xu Cui, Cynthia Roberts, Hao Jiang, Hua Teng, Michael Chopp
• Journal: Neuroscience letters (Neurosci Lett) Vol. 404 Issue 1-2 Pg. 28-32 (Aug 14 2006) ISSN: 0304-3940 [Print] Ireland
• PMID: 16762501 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Angiopoietin-1
• Nitric Oxide Donors
• Nitroso Compounds
• RNA, Messenger
• 2,2'-(hydroxynitrosohydrazono)bis-ethanamine
• Receptor, TIE-2

Topics

• Angiopoietin-1 (genetics)
• Animals
• Gene Expression Regulation
• Male
• Middle Cerebral Artery
• Nitric Oxide Donors (pharmacology)
• Nitroso Compounds (pharmacology)
• RNA, Messenger (genetics)
• Rats
• Rats, Wistar
• Receptor, TIE-2 (genetics)
• Stroke (genetics, physiopathology)