Acute pancreatitis (AP) is an inflammatory disease of the pancreas characterized by local
inflammation and extrapancreatic effects such as
lung injury. Secretory
phospholipases A(2) (PLA(2)s) have been implicated in triggering AP, but their exact role to evoke AP is largely unknown. Therefore, we have tested the ability of
sPLA(2)s to induce AP in rats, using
venom sPLA(2)s with residual or high enzymatic activity (
bothropstoxin-II and Naja mocambique mocambique
venom PLA(2), respectively), as well as
sPLA(2) devoid of catalytic activity (
piratoxin-I). The injection of Naja m. mocambique
venom PLA(2),
bothropstoxin-II or
piratoxin-I (300 microg/kg each) into the common bile duct increased significantly the pancreatic plasma extravasation and
myeloperoxidase activity. The lung
myeloperoxidase and serum
amylase were also increased for all groups, although the Naja mocambique mocambique
venom PLA(2) induced higher lung
myeloperoxidase and serum
amylase values, compared with
piratoxin-I and/or
bothropstoxin-II. Histopathology of pancreas and lungs in
piratoxin-I-injected rats showed interstitial oedema in both tissues, and neutrophil infiltration with acinar cell
necrosis in pancreas. In conclusion,
sPLA(2)s induce AP in rats and the catalytic activity is not essential to induce the local effects in pancreas, although it appears to contribute partly to the remote
lung injury.