Antitumor activity of a fusion of esophageal carcinoma cells with dendritic cells derived from cord blood.
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| Abstract |
The aim of this experiment was to develop a cytotoxic cancer vaccine (EC109-DC) prepared by fusions of esophageal carcinoma cells with dendritic cells derived from cord blood and to study the biological characteristics and resultant induction of antitumor immunity. CD34+ hematopoietic stem cells were isolated from cord blood using a CD34+ Progenitor Cell Isolation Kit by magnetic cell sorting system (MACS). CD34+ cells were incubated with rhGM-CSF, rhTNF-alpha and rhSCF for 2 weeks as DC (dendritic cells), and then by PEG-3600 to fuse with an esophageal carcinoma cell line. Selection with MACS marked with HLA-DR MicroBeads generated EC109-DC. Phenotypes and proliferation were analyzed by flow cytometry and cell culture in vitro. The lymphocyte proliferation reaction and CTL cytotoxicity were examined by MTT assay. The EC109-DC cells could proliferate slowly in vitro and highly expressed CD80, CD83 and CD86. The lymphocyte proliferation reaction and specific cytotoxicity against EC109 induced by EC109-DC cells were significantly higher than in control groups (p < 0.05). EC109-DC cells obtained by PEG fusion acquired the immuno-stimulating phenotype and could significantly stimulate the lymphocyte proliferation reaction and CTL activity. The results of this research provide the basis for materials to develop the DC-based vaccine against esophageal carcinoma.
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| Authors | Guanghua Guo, Suzuan Chen, Juan Zhang, Lili Luo, Jing Yu, Hongmei Dong, Hong Xu, Zhongjing Su, Libiao Wu |
| Journal | Vaccine (Vaccine) Vol. 23 Issue 45 Pg. 5225-30 (Nov 01 2005) ISSN: 0264-410X [Print] Netherlands |
| PMID | 16171908 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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