Nuclear sphingomyelin-synthase and protein kinase C delta in melanoma cells.

Abstract

The aim of this research is to study the influence of protein kinase C delta on the nuclear phospholipids metabolism. Murine and human melanoma cells, in which overexpression of protein kinase delta was induced, were used. After purification of the nuclei, the phosphatidylcholine-dependent phospholipase C, sphingomyelin-synthase, and sphingomyelinase activities were measured. The results showed that the nuclear sphingomyelin-synthase activity increased and sphingomyelinase activity decreased in the protein kinase C delta overexpressive cells with respect to the controls. As a consequence, the ceramide pool decreased and diacylglycerol pool increased; this effect was not due to the phosphatidylcholine-dependent phospholipase C activity that did not change. The inhibition of sphingomyelinase could be due to protein kinase C delta as well as to existence of a sort of nuclear self-regulation between sphingomyelin-synthase and sphingomyelinase. The possible role of nuclear sphingomyelin-synthase in cell proliferation is discussed.

• Authors: Elisabetta Albi, Caterina A M La Porta, Samuela Cataldi, Mariapia Viola Magni
• Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 438 Issue 2 Pg. 156-61 (Jun 15 2005) ISSN: 0003-9861 [Print] United States
• PMID: 15907783 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ceramides
• Phosphatidylcholines
• Prkcd protein, mouse
• PRKCD protein, human
• Protein Kinase C
• Protein Kinase C-delta
• Transferases (Other Substituted Phosphate Groups)
• phosphatidylcholine-ceramide phosphocholine transferase
• Type C Phospholipases
• Sphingomyelin Phosphodiesterase

Topics

• Animals
• Cell Line, Tumor
• Cell Nucleus (enzymology, metabolism)
• Cell Proliferation
• Ceramides (metabolism)
• Humans
• Melanoma (enzymology, metabolism)
• Mice
• Phosphatidylcholines (metabolism)
• Protein Kinase C (biosynthesis, metabolism, physiology)
• Protein Kinase C-delta
• Sphingomyelin Phosphodiesterase (metabolism)
• Transfection
• Transferases (Other Substituted Phosphate Groups) (biosynthesis, physiology)
• Type C Phospholipases (metabolism)