DNA fragment encoding human IL-1beta 163-171 peptide enhances the immune responses elicited in mice by DNA vaccine against foot-and-mouth disease.

Abstract

DNA vaccine has been tested for protection against foot-and-mouth disease. However, the relatively low efficacy of DNA vaccine in inducing immune responses in large animals has restricted its practical use. Interleukin-1 plays an essential role in amplifying both the cellular and humoral immune responses to foreign antigens, and may therefore represent a good candidate as an adjuvant of DNA vaccines. Since the inflammatory activity of IL-I may restrict its application in DNA vaccine treatment, we explored the possibilities of augmenting immune responses without unwanted inflammatory effects using the IL-1beta fragment (amino acids (aa) 163-171), which is essential for IL-1 receptor-1 binding. The DNA fragment encoding the human IL-1beta fragment (aa 163-171) was fused to foot-and-mouth disease virus (FMDV) DNA vaccine, and injected into mice to analyse its immune response. Compared with control mice receiving FMDV DNA vaccine alone, significant increases in the FMDV-specific antibody response and also in T cell proliferation were observed in mice receiving IL-1beta (163-171)-FMDV. These results suggested that DNA fragment encoding IL-1beta 163-171 peptide might represent a good candidate for an adjuvant of FMDV DNA vaccine.

• Authors: H J Shao, L Chen, Y B Su
• Journal: Veterinary research communications (Vet Res Commun) Vol. 29 Issue 1 Pg. 35-46 (Jan 2005) ISSN: 0165-7380 [Print] Netherlands
• PMID: 15727290 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Interleukin-1
• Interleukin-1beta
• Peptide Fragments
• Recombinant Fusion Proteins
• Vaccines, DNA
• Viral Vaccines
• interleukin-1beta (163-171)

Topics

• Adjuvants, Immunologic (genetics)
• Amino Acid Sequence
• Animals
• Foot-and-Mouth Disease (immunology, prevention & control)
• Humans
• Interleukin-1 (genetics, immunology)
• Interleukin-1beta
• Male
• Mice
• Mice, Inbred BALB C
• Peptide Fragments (genetics, immunology)
• Plasmids
• Recombinant Fusion Proteins (immunology)
• Vaccines, DNA (immunology)
• Viral Vaccines (immunology)