Abstract |
This study was carried out to investigate the transient ischemia-induced changes of neurofilament 200 kDa (NF-200) immunoreactivity and protein content in the gerbil lateral olfactory tract (LOT) after 5 min of transient forebrain ischemia. Weak NF-200 immunoreactivity was detectable in the LOT in the sham-operated group. In this group, a few somata of mitral cells showed weak NF-200 immunoreactivity. One day after transient ischemia, NF-200 immunoreactivity in the LOT was increased significantly. NF-200 immunoreactivity in the LOT by 15 days after ischemia was similar to that in the 1 day post-ischemic group. In this time period, strong NF-200 immunoreactivity was expressed in the mitral cell processes, but the immunoreactivity in the mitral cell somata was significantly decreased. Thereafter, NF-200 immunoreactivity in the LOT was decreased significantly by 30 days after ischemic insult. At this time after ischemia, NF-200 immunoreactivity in the mitral cell dendrites was significantly decreased. The result of Western blot study showed that the pattern of NF-200 expression was similar to that of immunohistochemistry after ischemia-reperfusion. Our result suggests that changes of NF-200 protein in the gerbil LOT may be related to response to ischemic damage and that the axonal transport followed transient ischemia may be disturbed.
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Authors | Hyeon Yong Lee, In Koo Hwang, Un San Koh, Jae Chul Lee, Dae Ho Kim, Jung Hwa Kim, Chang Ho Kim, Ki-Yeon Yoo, Tae-Cheon Kang, Baek Hwang, Moo Ho Won |
Journal | Brain research
(Brain Res)
Vol. 1036
Issue 1-2
Pg. 202-7
(Mar 02 2005)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 15725419
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neurofilament Proteins
- neurofilament protein H
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Topics |
- Animals
- Axonal Transport
(physiology)
- Axons
(metabolism)
- Cytoskeleton
(metabolism)
- Dendrites
(metabolism)
- Disease Models, Animal
- Gerbillinae
- Immunohistochemistry
- Ischemic Attack, Transient
(metabolism, physiopathology)
- Male
- Neurofilament Proteins
(metabolism)
- Olfactory Bulb
(metabolism, physiopathology)
- Olfactory Pathways
(metabolism, physiopathology)
- Time Factors
- Up-Regulation
(physiology)
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