Abstract |
In order to develop a new skin whitening agent, safflower (Carthamus tinctorius L.) seeds were evaluated for melanogenesis inhibitory activity and its active principles were identified following activity-guided isolation. The 80% aqueous methanol extract and ethyl acetate fraction from safflower seeds showed a significant inhibition for mushroom tyrosinase. Three active compounds, N-feruloylserotonin, N-(p-coumaroyl)serotonin, and acacetin, were isolated from the ethyl acetate fraction as the active principles. Compared with arbutin (IC50=0.223 mM), the IC50 values of these compounds were 0.023, 0.074, and 0.779 mM for N-feruloylserotonin, N-(p-coumaroyl)serotonin, and acacetin, respectively. It was also found that N-feruloylserotonin and N-(p-coumaroyl)serotonin strongly inhibited the melanin production of Streptomyces bikiniensis and B16 melanoma cells in comparison with a known melanogenesis inhibitor, arbutin.
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Authors | Jung Seop Roh, Ji Young Han, Jung Han Kim, Jae Kwan Hwang |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 27
Issue 12
Pg. 1976-8
(Dec 2004)
ISSN: 0918-6158 [Print] Japan |
PMID | 15577216
(Publication Type: Journal Article)
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Chemical References |
- Melanins
- Plant Extracts
- Monophenol Monooxygenase
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Topics |
- Animals
- Carthamus tinctorius
- Melanins
(antagonists & inhibitors, biosynthesis)
- Melanoma, Experimental
(metabolism)
- Mice
- Mice, Inbred C57BL
- Monophenol Monooxygenase
(antagonists & inhibitors, metabolism)
- Plant Extracts
(chemistry, isolation & purification, pharmacology)
- Seeds
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