Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G2-phase arrest.

Abstract

AIM:

To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402).

METHODS:

Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis.

RESULTS:

PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 mumol/L for 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h.

CONCLUSION:

Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.

• Authors: Hong-ying Chen, Cong-yi Zheng, Guo-lin Zou, Da-xing Xie, Jian-ping Gong
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 25 Issue 12 Pg. 1698-704 (Dec 2004) ISSN: 1671-4083 [Print] United States
• PMID: 15569418 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibiotics, Antineoplastic
• Cyclin A
• Cyclin B
• Peplomycin

Topics

• Antibiotics, Antineoplastic (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (metabolism, pathology)
• Cell Line, Tumor
• Cyclin A (metabolism)
• Cyclin B (metabolism)
• G1 Phase
• G2 Phase
• Humans
• Liver Neoplasms (metabolism, pathology)
• Peplomycin (pharmacology)