Glutamatergic neurotransmission modulates hypoxia-induced hyperventilation but not anapyrexia.

Abstract

The interaction between pulmonary ventilation (V E) and body temperature (Tb) is essential for O2 delivery to match metabolic rate under varying states of metabolic demand. Hypoxia causes hyperventilation and anapyrexia (a regulated drop in Tb), but the neurotransmitters responsible for this interaction are not well known. Since L-glutamate is released centrally in response to peripheral chemoreceptor stimulation and glutamatergic receptors are spread in the central nervous system we tested the hypothesis that central L-glutamate mediates the ventilatory and thermal responses to hypoxia. We measured V E and Tb in 40 adult male Wistar rats (270 to 300 g) before and after intracerebroventricular injection of kynurenic acid (KYN, an ionotropic glutamatergic receptor antagonist), alpha-methyl-4-carboxyphenylglycine (MCPG, a metabotropic glutamatergic receptor antagonist) or vehicle (saline), followed by a 1-h period of hypoxia (7% inspired O2) or normoxia (humidified room air). Under normoxia, KYN (N = 5) or MCPG (N = 8) treatment did not affect V E or Tb compared to saline (N = 6). KYN and MCPG injection caused a decrease in hypoxia-induced hyperventilation (595 +/- 49 for KYN, N = 7 and 525 +/- 84 ml kg-1 min-1 for MCPG, N = 6; P < 0.05) but did not affect anapyrexia (35.3 +/- 0.2 for KYN and 34.7 +/- 0.4 masculine C for MCPG) compared to saline (912 +/- 110 ml kg-1 min-1 and 34.8 +/- 0.2 masculine C, N = 8). We conclude that glutamatergic receptors are involved in hypoxic hyperventilation but do not affect anapyrexia, indicating that L-glutamate is not a common mediator of this interaction.

• Authors: P M de Paula, L G S Branco
• Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 37 Issue 10 Pg. 1581-9 (Oct 2004) ISSN: 0100-879X [Print] Brazil
• PMID: 15448881 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzoates
• Excitatory Amino Acid Antagonists
• alpha-methyl-4-carboxyphenylglycine
• Glutamic Acid
• Kynurenic Acid
• Glycine

Topics

• Animals
• Benzoates (pharmacology)
• Body Temperature (drug effects, physiology)
• Body Temperature Regulation
• Excitatory Amino Acid Antagonists (pharmacology)
• Glutamic Acid (drug effects, physiology)
• Glycine (analogs & derivatives, pharmacology)
• Hyperventilation (etiology)
• Hypoxia (complications)
• Injections, Intraventricular
• Kynurenic Acid (pharmacology)
• Male
• Rats
• Rats, Wistar