Expression of RUNX3 protein in human gastric mucosa, intestinal metaplasia and carcinoma.

Abstract

BACKGROUND:

Human runt-related transcription factor gene 3 (RUNX3) is considered as a possible candidate of tumour suppressor gene in human gastric carcinoma.

MATERIALS AND METHODS:

To investigate the RUNX3 protein expression in human gastric mucosa and carcinoma, we generated a polyclonal antibody, AS251, which recognized amino acid sequences from 251 to 266 of human RUNX3. The AS251 antibody was immunoreactive with only RUNX3 protein, but not with RUNX1 and RUNX2. The AS251-antibody was available for Western blotting and immunohistochemistry using paraffin-embedded specimens.

RESULTS:

Western-blot analysis revealed that three (MKN-1, -7 and -45) of six human gastric carcinoma cell lines variably expressed RUNX3 protein, consistent with the expression pattern of RUNX3 mRNA reported previously by Li et al. (Cell 2002;109:113-24). Immunohistochemistry disclosed RUNX3 protein in most chief cells and a few gastrin-containing G cells in normal mucosa, but not in intestinal metaplasia and carcinoma cells.

CONCLUSIONS:

These data suggest that RUNX3 may play a physiologic role in chief cells and G cells in gastric mucosa, and that suppression of RUNX3 expression in intestinal metaplasia and carcinoma of human stomach may be implicated in gastric carcinogenesis.

• Authors: M Osaki, M Moriyama, K Adachi, C Nakada, A Takeda, Y Inoue, H Adachi, K Sato, M Oshimura, H Ito
• Journal: European journal of clinical investigation (Eur J Clin Invest) Vol. 34 Issue 9 Pg. 605-12 (Sep 2004) ISSN: 0014-2972 [Print] England
• PMID: 15379759 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Core Binding Factor Alpha 3 Subunit
• DNA-Binding Proteins
• Runx3 protein, human
• Transcription Factors

Topics

• Amino Acid Sequence
• Blotting, Western
• Carcinoma (metabolism)
• Cell Line, Tumor
• Core Binding Factor Alpha 3 Subunit
• DNA-Binding Proteins (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Gastric Mucosa (metabolism)
• Humans
• Metaplasia (metabolism)
• Stomach (pathology)
• Stomach Neoplasms (metabolism)
• Transcription Factors (metabolism)