Abstract |
Recent efforts to design an human immunodeficiency virus type 1 (HIV-1) vaccine candidate have focused on means of eliciting anti-viral T-cell responses. We tried to improve the immunogenicity of DNA vaccines by designing hybrid DNA constructs encoding hepatitis B surface antigen ( HBsAg) fused to antigenic domains of simian/human immunodeficiency virus (SHIV 89.6P). Immunisation with hybrid DNA induced both effector and long-lasting precursor T-cells. Following boosting with a recombinant modified vaccinia Ankara (rMVA) producing full-length SIV and HIV antigens, it appeared that priming with hybrid DNA had increased virus-specific T-cell responses in terms of both the number of virus-specific IFN-gamma-secreting T-cells and virus-specific lymphoproliferation. After intrarectal challenge with SHIV 89.6P, immunised animals demonstrated early control of SHIV 89.6P replication and stable CD4+ T-cell counts.
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Authors | Anne-Laure Puaux, Delphine Marsac, Stéphane Prost, Mandal K Singh, Patricia Earl, Bernard Moss, Roger Le Grand, Yves Riviere, Marie-Louise Michel |
Journal | Vaccine
(Vaccine)
Vol. 22
Issue 27-28
Pg. 3535-45
(Sep 09 2004)
ISSN: 0264-410X [Print] Netherlands |
PMID | 15315833
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AIDS Vaccines
- DNA Probes
- Hepatitis B Surface Antigens
- SAIDS Vaccines
- Vaccines, DNA
- Vaccines, Subunit
- Interferon-gamma
- DNA
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Topics |
- AIDS Vaccines
(therapeutic use)
- Animals
- Area Under Curve
- CD4-Positive T-Lymphocytes
(immunology)
- Cell Division
(drug effects)
- Cytomegalovirus
(genetics)
- DNA
(genetics, immunology)
- DNA Probes
- Enzyme-Linked Immunosorbent Assay
- Genetic Vectors
- HIV
(immunology)
- Hepatitis B Surface Antigens
(genetics, immunology)
- Immunization, Secondary
- Interferon-gamma
- Kinetics
- Macaca mulatta
- Reverse Transcriptase Polymerase Chain Reaction
- SAIDS Vaccines
(therapeutic use)
- Simian Immunodeficiency Virus
(immunology)
- T-Lymphocytes
(drug effects, immunology)
- Vaccines, DNA
(therapeutic use)
- Vaccines, Subunit
(therapeutic use)
- Vaccinia
(immunology)
- Virus Replication
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