Effects of AGEs on oxidation stress and antioxidation abilities in cultured astrocytes.

Abstract

OBJECTIVE:

To investigate whether two kinds of in vitro prepared advanced glycation end products (AGEs), Glu-BSA and Gal-BSA, could change oxidation stress and anti-oxidation abilities in astrocytes, and thus might contribute to brain injury.

METHODS:

Changes of GSH, MDA, SOD, MAO-B, nitric oxide were measured after AGEs treatment.

RESULTS:

Both 0.1 g/L Glu-BSA and Gal-BSA could slightly decrease GSH level, while 1 g/L of them significantly decreased GSH level by 35% and 43% respectively. The MDA levels of both 1 g/L AGEs treated groups (306 +/- 13 and 346 +/- 22) were higher than that of the normal group (189 +/- 18), which could be inhibited by free radical scavenger NAC. The SOD activities of both 1 g/L AGEs treated groups (67.0 +/- 5.2 and 74.0 +/- 11.0) were lower than that of the normal group (85.2 +/- 8.0). Both 0.1 g/L AGEs could slightly increase the activity of MAO-B, while 1 g/L of them could increase MAO-B activity by 1.5 and 1.7 folds respectively. Both AGEs stimulation could produce NO level by 1.7 and 2 folds respectively.

CONCLUSION:

Enhanced levels of astrocytic oxidation stress and decrease of antioxidation abilities may contribute to, at least partially, the detrimental effects of AGEs in neuronal disorders and aging brain.

• Authors: Jian-Ming Jiang, Zhen Wang, Dian-Dong Li
• Journal: Biomedical and environmental sciences : BES (Biomed Environ Sci) Vol. 17 Issue 1 Pg. 79-86 (Mar 2004) ISSN: 0895-3988 [Print] China
• PMID: 15202867 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycation End Products, Advanced
• advanced glycation end products-bovine serum albumin
• Serum Albumin, Bovine
• Nitric Oxide
• Malondialdehyde
• Superoxide Dismutase
• Monoamine Oxidase
• Glutathione

Topics

• Animals
• Astrocytes (drug effects, enzymology, metabolism)
• Cattle
• Cells, Cultured
• Cerebral Cortex (cytology)
• Glutathione (metabolism)
• Glycation End Products, Advanced (pharmacology)
• Malondialdehyde (metabolism)
• Monoamine Oxidase (metabolism)
• Nitric Oxide (metabolism)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Serum Albumin, Bovine (pharmacology)
• Superoxide Dismutase (metabolism)