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[Activity of the key enzymes in xylose-assimilating yeasts at different rates of oxygen transfer to the fermentation medium].

Abstract
The activities of xylitol dehydrogenase and xylose reductase in the yeasts Candida shehatae, C. didensiae, C. intermediae, C. tropicalis, Kluyveromyces marxianus, Pichia stipitis, P. guillermondii, Pachysolen tannophilus, and Torulopsis molishiama were studied at different oxygen transfer rates (OTRs) to the fermentation medium (0, 5, and 140 mmol O2/(1 h)). The activities of these enzymes were maximum in the yeasts P. stipitis and C. shehatae. The xylitol dehydrogenase of all the yeasts was NAD-dependent, irrespective of the intensity of aeration. The xylose reductase of the yeasts C. didensiae, C. intermediae, C. tropicalis, Kl. marxianus, P. guillermondii, and T. molishiama was NADPH-dependent, whereas the xylose reductase of P. stipitis, C. shehatae, and Pa. tannophilus was specific for both NADPH and NADH. The effect of OTR on the activities of the different forms of xylitol dehydrogenase and xylose reductase in the xylose-assimilating yeasts is discussed.
AuthorsE N Iablochkova, O I Bolotnikova, N P Mikhaĭlova, N N Nemova, A I Ginak
JournalMikrobiologiia (Mikrobiologiia) 2004 Mar-Apr Vol. 73 Issue 2 Pg. 163-8 ISSN: 0026-3656 [Print] Russia (Federation)
Vernacular TitleAktivnost' kliuchevykh fermentov ksilozoassimiliruiushchikh drozhzheĭ pri razlichnoĭ skorosti postupleniia kisloroda v fermentatsionnuiu sredu.
PMID15198025 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Culture Media
  • NAD
  • NADP
  • Xylose
  • Sugar Alcohol Dehydrogenases
  • Aldehyde Reductase
  • D-Xylulose Reductase
  • Oxygen
Topics
  • Aerobiosis
  • Aldehyde Reductase (metabolism)
  • Candida (metabolism)
  • Culture Media
  • D-Xylulose Reductase
  • Fermentation
  • NAD (metabolism)
  • NADP (metabolism)
  • Oxygen (metabolism)
  • Pichia (metabolism)
  • Species Specificity
  • Sugar Alcohol Dehydrogenases (metabolism)
  • Xylose (metabolism)
  • Yeasts (enzymology, growth & development)

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