Ionspray mass spectrometry of marine toxins. IV. Determination of diarrhetic shellfish poisoning toxins in mussel tissue by liquid chromatography/mass spectrometry.
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| Abstract |
An improved liquid chromatographic/mass spectrometric (LC/MS) method utilizing gradient elution and ion-spray ionization is described for the sensitive determination of okadaic acid and dinophysistoxin-1, the principal toxins implicated in cases of diarrhetic shellfish poisoning. The method was used to confirm the presence of both toxins, together with a recently identified isomer of okadaic acid, dinophysistoxin-2, in various samples of cultivated blue mussels (Mytilus edulis) from Canadian and European waters. The method provided a mass detection limit of 0.4 ng for each toxin, thus allowing detection of 40 ng per g of whole mussel tissue (or approximately 10 ng/g if only the digestive glands were used in the assay). Quantitative results obtained by LC/MS were in good agreement with those obtained by derivatization and high-performance liquid chromatography with fluorescence detection.
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| Authors | S Pleasance, M A Quilliam, J C Marr |
| Journal | Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom) Vol. 6 Issue 2 Pg. 121-7 (Feb 1992) ISSN: 0951-4198 [Print] England |
| PMID | 1504340 (Publication Type: Journal Article) |
| Chemical References |
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