Abstract | OBJECTIVE: Inducible heat shock protein 72 (HSP 72) preserves myocardial function and prevents apoptosis. We investigated the expression and localization of HSP 72 and apoptosis in our previously described new model of multiple organ failure. DESIGN: MEASUREMENTS AND RESULTS: Hearts were analyzed by light microscopy, Western blots, immunohistochemistry, and TUNEL staining. HSP 72 expression was approximately threefold increased in NMH+ENDO compared with the other groups ( p<0.05) and was localized mainly in left ventricular cardiomyocytes. HSP 72 was elevated in animals with norfenefrine-refractory shock compared to survivors ( p=0.015). TUNEL-positive cells in the left ventricle were significantly elevated in the NMH+ENDO group ( p=0.05) and correlated with HSP 72 expression (r=0.51, p=0.018). HSP 72 correlated positively with heart rate (r=0.76, p<0.0001), the prefinal hourly dose of norfenefrine (r=0.88, p<0.0001), and negatively with left ventricular stroke work index (r=-0.52, p=0.028). Double staining revealed TUNEL-positive cells with and without HSP 72 expression. Micronecroses were only detectable in NMH and NMH+ENDO without intergroup difference or correlations with hemodynamics. CONCLUSION:
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Authors | Hideo A Baba, Jeremias Wohlschlaeger, Henning D Stubbe, Florian Grabellus, Hugo Van Aken, Klaus J Schmitz, Friedrich Otterbach, Kurt W Schmid, Christian August, Bodo Levkau, Frank Hinder |
Journal | Intensive care medicine
(Intensive Care Med)
Vol. 30
Issue 7
Pg. 1405-13
(Jul 2004)
ISSN: 0342-4642 [Print] United States |
PMID | 14985962
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HSP72 Heat-Shock Proteins
- Heat-Shock Proteins
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Topics |
- Animals
- Apoptosis
- Blotting, Western
- Disease Models, Animal
- HSP72 Heat-Shock Proteins
- Heat-Shock Proteins
(analysis, metabolism)
- Immunohistochemistry
- In Situ Nick-End Labeling
- Multiple Organ Failure
(metabolism, pathology)
- Myocardium
(chemistry, metabolism, pathology)
- Random Allocation
- Sheep
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