Interleukin (IL)-4 and to a lesser extent either IL-13 or interferon-gamma regulate the production of eotaxin-2/CCL24 in nasal polyps.

Abstract

BACKGROUND:

Eotaxin-2/CCL24 is a potent eosinophil attractant that has been implicated in the recruitment of eosinophils in allergic disease. We have investigated whether the cytokines interleukin (IL)-4, IL-13, and interferon (IFN)-gamma regulate eotaxin-2/CCL24 in nasal polyps.

METHODS:

Nasal polyps were cultured in the presence of the cytokines described above and the concentration of eotaxin-2/CCL24 was measured in the culture supernatant.

RESULTS:

IL-4 was found to be the major stimulus for eotaxin-2/CCL24 production from nasal polyps followed by IL-13 and IFN-gamma. IL-4 induced eotaxin-2/CCL24 in a dose-dependent manner with concentrations as low as 0.1 ng/ml being able to induce eotaxin-2/CCL24. By immunohistochemistry, eotaxin-2/CCL24 immunoreactivity was localized to mononuclear cells in the IL-4 stimulated nasal polyp tissue. Interestingly, nasal turbinates obtained from patients suffering from nonallergic rhinitis (vasomotor rhinitis) were also found to release eotaxin-2/CCL24 both spontaneously and following cytokine stimulation with IL-4 and IFN-gamma being major inducers of this cytokine.

CONCLUSIONS:

All together these findings suggest that Th1 and Th2 cytokines may regulate eotaxin-2/CCL24 production in nasal polyps and nonallergic rhinits.

• Authors: D Lezcano-Meza, B Dávila-Dávila, A Vega-Miranda, M C Negrete-García, L M Teran
• Journal: Allergy (Allergy) Vol. 58 Issue 10 Pg. 1011-7 (Oct 2003) ISSN: 0105-4538 [Print] Denmark
• PMID: 14510718 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCL24 protein, human
• Chemokine CCL24
• Chemokines, CC
• Interleukin-13
• Interleukin-4
• Interferon-gamma

Topics

• Adult
• Cells, Cultured
• Chemokine CCL24
• Chemokines, CC (biosynthesis, immunology)
• Dose-Response Relationship, Drug
• Female
• Humans
• Interferon-gamma (pharmacology, physiology)
• Interleukin-13 (pharmacology, physiology)
• Interleukin-4 (pharmacology, physiology)
• Kinetics
• Leukocytes, Mononuclear (immunology)
• Middle Aged
• Nasal Polyps (immunology, pathology)