Abstract |
Previous studies in our laboratory have indicated a role for free radical participation in magnesium deficiency cardiomyopathy. We have demonstrated the ability of various antioxidant drugs and nutrients to protect against magnesium deficiency-induced myocardial injury. In this study, we have examined erythrocytes from normal and magnesium-deficient animals and compared their susceptibility to an in vitro oxidative stress. Syrian male hamsters were placed on either magnesium-deficient or magnesium-supplemented diets. Animals from each group also received vitamin E in doses of 10 and 25 mg as subcutaneous implants. Erythrocytes obtained after 14 days on the diet were exposed to an exogenous hydroxyl (. OH) radical generating system ( dihydroxyfumarate not equal to Fe3+ ADP) at 37 degrees C for 20 min. Erythrocyte crenation was observed and quantified by scanning electron microscopy. Lipid peroxidation, hemolysis (%), and intracellular glutathione levels were determined. In addition, serum lipid changes and membrane phospholipids were characterized. Our data demonstrate that erythrocytes from magnesium-deficient animals are more susceptible to free radical injury, supporting our hypothesis that magnesium deficiency reduces the threshold antioxidant capacity.
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Authors | A M Freedman, I T Mak, R E Stafford, B F Dickens, M M Cassidy, R A Muesing, W B Weglicki |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 262
Issue 6 Pt 1
Pg. C1371-5
(Jun 1992)
ISSN: 0002-9513 [Print] United States |
PMID | 1319674
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Chlorides
- Ferric Compounds
- Fumarates
- Hydroxides
- Iron Chelating Agents
- Membrane Lipids
- Phospholipids
- Triglycerides
- Superoxides
- dihydroxyfumarate
- Hydroxyl Radical
- Malondialdehyde
- Adenosine Diphosphate
- Cholesterol
- Glutathione
- ferric chloride
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Topics |
- Adenosine Diphosphate
(pharmacology)
- Animals
- Chlorides
- Cholesterol
(blood)
- Cricetinae
- Erythrocyte Membrane
(chemistry)
- Erythrocytes
(drug effects, physiology, ultrastructure)
- Ferric Compounds
(pharmacology)
- Fumarates
(pharmacology)
- Glutathione
(blood)
- Hemolysis
- Hydroxides
(blood)
- Hydroxyl Radical
- In Vitro Techniques
- Iron Chelating Agents
(pharmacology)
- Lipid Peroxidation
- Magnesium Deficiency
(blood)
- Male
- Malondialdehyde
(blood)
- Membrane Lipids
(blood)
- Mesocricetus
- Microscopy, Electron, Scanning
- Phospholipids
(blood)
- Reference Values
- Superoxides
(blood)
- Triglycerides
(blood)
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