Accelerated alveolar bone loss in mice lacking interleukin-10.

Abstract

Interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. We hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. Male interleukin-10(-/-) mice, on 129/SvEv and C57BL/6J background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. Immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. Interleukin-10(-/-) mice had significantly greater alveolar bone loss than interleukin-10(+/+) mice (p = 0.006). The 30-40% greater alveolar bone loss in interleukin-10(-/-) mice was evident in both strains, with C57BL/6J interleukin-10(-/-) mice exhibiting the most bone loss. Immunoblotting revealed distinct interleukin-10(-/-) serum reactivity against Bacteroides vulgatus, B. fragilis, Prevotella intermedia, and, to a lesser extent, against B. forsythus. The results of the present study suggest that lack of interleukin-10 leads to accelerated alveolar bone loss.

• Authors: A Al-Rasheed, H Scheerens, D M Rennick, H M Fletcher, D N Tatakis
• Journal: Journal of dental research (J Dent Res) Vol. 82 Issue 8 Pg. 632-5 (Aug 2003) ISSN: 0022-0345 [Print] United States
• PMID: 12885849 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Interleukin-10

Topics

• Alveolar Bone Loss (classification, immunology, microbiology)
• Animals
• Antibodies, Bacterial (blood)
• Bacteroides (immunology)
• Bacteroides fragilis (immunology)
• Immunoblotting
• Interleukin-10 (blood, immunology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Inbred Strains
• Porphyromonas gingivalis (immunology)
• Prevotella intermedia (immunology)
• Statistics, Nonparametric