Phloxine B effect on immature stages of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann).

Abstract

A laboratory bioassay was developed to determine both the chemical toxicity and the phototoxicity of the xanthene dye, phloxine B (D&C Red No 28), to the immature stages of the Mediterranean fruit fly, Certitis capitata (Wiedemann). An additional goal was to find out which main tissues are affected first. A low, but significant, level of toxicity was observed when the insects were maintained in the dark: at the point of adult ecdysis, the LC50 was 11.03 mM. As expected, after 8-h exposure of late larva III to light, a high level of mortality was produced (LC50 at ecdysis: 0.45 mM) as a dose-dependent function of dye concentration. At sublethal concentrations of the dye, the surviving insects showed a number of physiological abnormalities. Phloxine B appeared to mainly affect the larval longitudinal muscles as well as the abdominal muscles of ecdysing adults, giving rise to abnormal puparia and failed adult ecdysis, respectively. Moreover, a significant phloxine B-dependent delay in the jumping of surviving larvae for dispersal was documented. This could be attributed to a delay in attaining a threshold weight for jumping and/or to abnormalities in neuromuscular coordination, thus reinforcing the idea of pleiotropic effects of the dye.

• Authors: Jimena Berni, Alejandro Rabossi, Luis A Quesada-Allué
• Journal: Journal of economic entomology (J Econ Entomol) Vol. 96 Issue 3 Pg. 662-8 (Jun 2003) ISSN: 0022-0493 [Print] England
• PMID: 12852602 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Eosine I Bluish

Topics

• Animals
• Ceratitis capitata (drug effects, growth & development)
• Darkness
• Eosine I Bluish (toxicity)
• Larva (drug effects, physiology)
• Lethal Dose 50
• Life Cycle Stages (drug effects)
• Light
• Motor Activity (drug effects)