Abstract |
Two novel stem cell factor (SCF) dependent human mast cell lines, designated LAD 1 and 2, were established from bone marrow aspirates from a patient with mast cell sarcoma/ leukemia. LAD 1 and 2 cells have the ultrastructural features of human mast cells, and express FcepsilonRI, CD4, 9, 13, 14, 22, 31, 32, 45, 64, 71, 103, 117, 132, CXCR4 (CD184), CCR5 (CD195); and intracytoplasmic histamine, tryptase and chymase. LAD 1 and 2 do not exhibit activating mutations at codon 816 of c-kit. Both LAD 1 and 2 release beta-hexosaminidase following FcepsilonRI or FcgammaRI aggregation. The availability of these cell lines offers an unparalleled circumstance to examine the biology of human mast cells.
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Authors | Arnold S Kirshenbaum, Cem Akin, Yalin Wu, Menachem Rottem, Julie P Goff, Michael A Beaven, V Koneti Rao, Dean D Metcalfe |
Journal | Leukemia research
(Leuk Res)
Vol. 27
Issue 8
Pg. 677-82
(Aug 2003)
ISSN: 0145-2126 [Print] England |
PMID | 12801524
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Receptors, IgE
- Receptors, IgG
- Stem Cell Factor
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Topics |
- Adult
- Bone Marrow Cells
(pathology)
- Cell Division
- Cell Line
- Dimerization
- Humans
- Immunophenotyping
- Karyotyping
- Leukemia, Mast-Cell
(enzymology, genetics, pathology)
- Male
- Mast Cells
(enzymology, pathology)
- Mast-Cell Sarcoma
(enzymology, genetics, pathology)
- Mutation
- Receptors, IgE
(metabolism)
- Receptors, IgG
(metabolism)
- Stem Cell Factor
(pharmacology)
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