5-Lipoxygenase knockout mice exhibit a resistance to pleurisy and lung injury caused by carrageenan.
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| Abstract |
In the present study, by comparing the responses in wild-type (WT) mice and mice lacking [knockout (KO)] the 5-lipoxygenase (5-LO), we investigated the role played by 5-LO in the development of acute inflammation. When compared with carragenan-treated 5-LOWT mice, 5-LOKO mice, which had received carrageenan, exhibited a reduced degree of pleural exudation, polymorphonuclear cell migration. Lung myeloperoxidase activity, an index of neutrophil infiltration, was significantly reduced in 5-LOKO mice in comparison with 5-LOWT. Lung-tissue sections from carrageenan-treated 5-LOWT mice showed positive staining for intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and E-selectin, which were mainly localized around vessels. The intensity and degree of ICAM-1, VCAM-1, P-selectin, and E-selectin were markedly reduced in tissue section from carrageenan-5-LOKO mice, which also improved the histological status of the inflamed lungs. Taken together, our results clearly demonstrate that 5-LO modulates neutrophil infiltration in the acute lung inflammation.
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| Authors | Salvatore Cuzzocrea, Antonietta Rossi, Ivana Serraino, Emanuela Mazzon, Rosanna Di Paola, Laura Dugo, Tiziana Genovese, Barbara Calabrò, Achille P Caputi, Lidia Sautebin |
| Journal | Journal of leukocyte biology (J Leukoc Biol) Vol. 73 Issue 6 Pg. 739-46 (Jun 2003) ISSN: 0741-5400 [Print] United States |
| PMID | 12773506 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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