Protection of chickens against highly lethal H5N1 and H7N1 avian influenza viruses with a recombinant fowlpox virus co-expressing H5 haemagglutinin and N1 neuraminidase genes.

Abstract

Inactivated whole avian influenza virus (AIV) vaccine provides protection against homologous haemagglutinin (HA) subtype virus, but poor protection against a heterologous HA virus. Moreover, it induces chickens to produce antibodies to cross-reactive antigens, especially nucleoprotein, which is limits AIV serological surveillance. In this study, a recombinant fowlpox virus co-expressing HA (H5 subtype) and NA (NI subtype)genes of AIV was evaluated for its ability to protect chickens against intramuscular challenge with a lethal dose of highly pathogenic (HP) AIV. Susceptible chickens were also vaccinated by wing-web puncture with the parent fowlpox vaccine virus. Following challenge 4 weeks later with HPAIV, all chickens vaccinated with recombinant virus were protected, while the chickens vaccinated with either the unaltered parent fowlpox vaccine virus or unvaccinated controls experienced 100% mortality following challenge. This protection was accompanied by the high levels of specific antibody to the respective components of the recombinant vaccine. The above results showed that rFPV-HA-NA could be a potential vaccine to replace current inactivated vaccines for preventing AI.

• Authors: Chuan-Ling Qiao, Kang-Zhen Yu, Yong-Ping Jiang, Yong-Qing Jia, Guo-Bin Tian, Ming Liu, Guo-Hua Deng, Xiu-Rong Wang, Qing-Wen Meng, Xiu-Ying Tang
• Journal: Avian pathology : journal of the W.V.P.A (Avian Pathol) Vol. 32 Issue 1 Pg. 25-32 (Feb 2003) ISSN: 0307-9457 [Print] England
• PMID: 12745375 (Publication Type: Clinical Trial, Journal Article)

Chemical References

• Antibodies, Viral
• DNA, Recombinant
• Hemagglutinin Glycoproteins, Influenza Virus
• Viral Vaccines
• hemagglutinin, avian influenza A virus
• Neuraminidase

Topics

• Animals
• Antibodies, Viral (immunology)
• Chickens
• DNA, Recombinant (genetics)
• Fowlpox virus (genetics, physiology)
• Gene Expression
• Genes, Viral (genetics)
• Hemagglutinin Glycoproteins, Influenza Virus (genetics, immunology)
• Influenza A Virus, H5N1 Subtype
• Influenza A virus (classification, genetics, immunology)
• Influenza in Birds (immunology, prevention & control)
• Neuraminidase (genetics, immunology)
• Poultry Diseases (immunology, prevention & control)
• Time Factors
• Viral Vaccines (genetics, immunology)