L-type voltage-gated calcium channel attends regulation of tyrosine phosphorylation of NMDA receptor subunit 2A induced by transient brain ischemia.

Abstract

To study the mechanism of the L-type voltage-gated calcium channel (L-VGCC) involved in neuronal injury induced by brain ischemia and reperfusion (I/R), transient (15 min) brain ischemia was induced by four-vessel occlusion of Sprague-Dawley (SD) rats. Tyrosine phosphorylation of NR2A and interaction of NR2A with Src and Pyk2 in hippocampus induced by brain ischemia and reperfusion (I/R) were determined by immunoprecipitation and immunoblot(ting). Tyrosine phosphorylation of NR2A in hippocampus was enhanced after I/R. Interaction of NR2A with Src and Pyk2, tyrosine phosphorylation and kinase activity of Src and Pyk2 also increased after I/R. All the increases were partly inhibited by L-VGCC antagonist nifedipine administered to rats 20 min prior to brain ischemia. The results suggested that increase of tyrosine phosphorylation of NR2A induced by I/R had a relation to activation of L-VGCC. Src and Pyk2 interacting with NR2A might also be involved in this regulation of the tyrosine phosphorylation of NR2A induced by I/R.

• Authors: Yong Liu, Xiao-Yu Hou, Guang-Yi Zhang, Tian-Le Xu
• Journal: Brain research (Brain Res) Vol. 972 Issue 1-2 Pg. 142-8 (May 16 2003) ISSN: 0006-8993 [Print] Netherlands
• PMID: 12711087 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium Channel Blockers
• Calcium Channels, L-Type
• Phosphorus Isotopes
• Receptors, N-Methyl-D-Aspartate
• Phosphotyrosine
• Tyrosine
• Protein-Tyrosine Kinases
• Focal Adhesion Kinase 2
• Ptk2b protein, rat
• src-Family Kinases
• Nifedipine
• N-methyl D-aspartate receptor subtype 2A

Topics

• Animals
• Brain Ischemia (metabolism)
• Calcium Channel Blockers (pharmacology)
• Calcium Channels, L-Type (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Focal Adhesion Kinase 2
• Hippocampus (drug effects, metabolism, physiopathology)
• Immunoblotting (methods)
• Male
• Nifedipine (pharmacology)
• Phosphorus Isotopes (metabolism)
• Phosphotyrosine (metabolism)
• Precipitin Tests (methods)
• Protein-Tyrosine Kinases (drug effects, metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptors, N-Methyl-D-Aspartate (drug effects, metabolism)
• Reperfusion (methods)
• Tyrosine (metabolism)
• src-Family Kinases (drug effects, metabolism)