Bio-artificial skin composed of gelatin and (1-->3), (1-->6)-beta-glucan.

Abstract

Porous scaffolds composed of gelatin and beta-glucan were prepared using the freeze-drying method. The scaffold had an inter-connected pore structure with average pore size of 90-150 microm. Results for the contact angle and cell attachment revealed that a high gelatin content was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures, because the gelatin had acidic residues, and arginine-glycine-aspartic acid groups. To prepare a stratified wound dressing to mimic the normal human skin, fibroblasts and keratinocyte cells were isolated from a child's foreskin, and were co-cultured in gelatin/beta-glucan scaffolds were cross-linked using 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride. An in vivo study showed that after 1 week, the artificial dermis containing the fibroblasts enhanced the re-epithelialization of a full-thickness skin defect rather than the acellular scaffold.

• Authors: Sang Bong Lee, Hyun Wook Jeon, Young Woo Lee, Young Moo Lee, Kang Won Song, Moon Hyang Park, Young Soo Nam, Hee Chang Ahn
• Journal: Biomaterials (Biomaterials) Vol. 24 Issue 14 Pg. 2503-11 (Jun 2003) ISSN: 0142-9612 [Print] Netherlands
• PMID: 12695077 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glucans
• Membranes, Artificial
• epiglucan
• Gelatin

Topics

• Animals
• Cell Adhesion
• Cell Division
• Child
• Culture Techniques (methods)
• Dermis (cytology, physiopathology)
• Extracellular Matrix (chemistry)
• Fibroblasts (transplantation)
• Freeze Drying
• Gelatin (chemistry)
• Glucans (chemistry)
• Humans
• Membranes, Artificial
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Skin, Artificial
• Tissue Engineering (methods)
• Treatment Outcome
• Wound Healing (physiology)
• Wounds, Penetrating (surgery)