Measurement of phosphatidylcholine hydroperoxide in mild ischemia-reperfusion injury in rat intestine.

Abstract

In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.

• Authors: Tsuyoshi Masuko, Yuji Funayama, Hiroo Naito, Kohei Fukushima, Chikashi Shibata, Ken-ichi Takahashi, Hitoshi Ogawa, Tatsuya Ueno, Akihiko Hashimoto, Teruo Miyazawa, Kiyotaka Nakagawa, Seiki Matsuno, Iwao Sasaki
• Journal: The Tohoku journal of experimental medicine (Tohoku J Exp Med) Vol. 198 Issue 4 Pg. 223-32 (Dec 2002) ISSN: 0040-8727 [Print] Japan
• PMID: 12630554 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Phosphatidylcholines
• Thiobarbituric Acid Reactive Substances
• phosphatidylcholine hydroperoxide
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Disease Models, Animal
• Immunohistochemistry
• Intercellular Adhesion Molecule-1 (metabolism)
• Intestinal Mucosa (metabolism, pathology)
• Male
• Neutrophils (pathology)
• Phosphatidylcholines (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology)
• Sensitivity and Specificity
• Thiobarbituric Acid Reactive Substances (metabolism)
• Time Factors