[Amplification, cloning and expression of a gene encoding hexose transporter of Plasmodium falciparum].

Abstract

OBJECTIVE:

To amplify, clone and express of a gene encoding hexose transporter of Plasmodium falcipuram (PfHT1) from Southern China isolate FCC1/HN for studing the immune of recombinant which protective from malaria parasite infection.

METHODS:

Cultivation of P. falciparum isolate FCC1/HN in vitro; extraction of genomic DNA from FCC1/HN using the alkali specific cleavage method; PCR amplification of PfHT1 and cloning into eukaryotic expression vector, pEGFPN3. The recombinant as introduced into mammalian cells, HEPG2 by using liposome-mediated transfection.

RESULTS:

The gene encoding PfHT1 was specifically amplified from the genomic DNA of P. falciparum isolate FCC1/HN. The size of amplified fragment was 1,516 base pair. The eukaryotic expression recombinant, pN3-HT1, was constructed and expressed steadily in the hepatocarcinoma cell lines, HEPG2.

CONCLUSION:

The gene encoding PfHT1 was successfully amplified and cloned. The pN3-HT1/HEPG2 cell line was built for expressing fusion protein of GFP-HT1.

• Authors: Q D Wei, X B Yu, L Ye, J Xu
• Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi) Vol. 18 Issue 6 Pg. 343-6 ( 2000) ISSN: 1000-7423 [Print] China
• PMID: 12567609 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Monosaccharide Transport Proteins
• Recombinant Fusion Proteins

Topics

• Animals
• Cloning, Molecular
• Gene Amplification
• Gene Expression
• Humans
• Monosaccharide Transport Proteins (biosynthesis, genetics)
• Plasmodium falciparum (genetics, immunology)
• Recombinant Fusion Proteins (biosynthesis, genetics)
• Transfection
• Tumor Cells, Cultured