Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry.

Abstract

Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFF(A) precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity.

• Authors: Odile Burlet-Schiltz, Honoré Mazarguil, Jean-Christophe Sol, Patrick Chaynes, Bernard Monsarrat, Jean-Marie Zajac, Anne Roussin
• Journal: FEBS letters (FEBS Lett) Vol. 532 Issue 3 Pg. 313-8 (Dec 18 2002) ISSN: 0014-5793 [Print] England
• PMID: 12482585 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligopeptides
• Peptides
• phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide

Topics

• Amino Acid Sequence
• Cerebrospinal Fluid (metabolism)
• Chromatography, High Pressure Liquid
• Humans
• Mass Spectrometry (methods)
• Molecular Sequence Data
• Oligopeptides (cerebrospinal fluid, chemistry)
• Peptides (chemistry)
• Radioimmunoassay
• Time Factors