Direct spectrofluorometry of diluted erythrocytes and plasma: a rapid diagnostic method in primary and secondary porphyrinemias.

Abstract

Primary and secondary porphyrinemias are characterized by the presence of several porphyrin species in erythrocytes and/or plasma. Acid solvent extraction of porphyrins from natural ligands can preclude observation of significant spectral differences apparent on direct spectrofluorometric examination of porphyrin complexes in tissue. Erythrocytes and hemoglobin-free plasma obtained from 13 patients with erythropoietic, 9 with lead intoxication, 5 with porphyria cutanea tarda, 3 with iron deficiency anemia, 1 with erythropoietic porphyria, and 10 normal persons were simply diluted and analyzed spectrofluorometrically. Characteristic porphyrin-ligand spectral patterns were consistently obtained for each group of specimens, allowing diagnostic application of the data.

• Authors: M B Poh-Fitzpatrick, A A Lamola
• Journal: The Journal of laboratory and clinical medicine (J Lab Clin Med) Vol. 87 Issue 2 Pg. 362-70 (Feb 1976) ISSN: 0022-2143 [Print] United States
• PMID: 1245797 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Porphyrins

Topics

• Anemia (blood)
• Anemia, Hypochromic (blood)
• Erythrocytes (analysis)
• Humans
• Lead Poisoning (blood)
• Plasma
• Porphyrias (blood)
• Porphyrins (blood)
• Spectrometry, Fluorescence (methods)