Abstract | BACKGROUND AND AIMS: To gain insight in intestinal epithelial proliferation, cell death, and gene expression during experimental colitis rats were treated with dextran sulfate sodium (DSS) for 7 days. MATERIALS AND METHODS: Proximal and distal colonic segments were excised on days 2, 5, 7, and 28. Epithelial proliferation, cell death, enterocyte gene expression ( carbonic anhydrase I (CA I) and goblet cell gene expression ( mucin, MUC2; trefoil factor 3, TFF3) were studied immunohistochemically and biochemically. RESULTS: Proliferative activity was decreased in the proximal and distal colon at the onset of disease (day 2). However, during active disease (days 5-7) epithelial proliferation was increased in the entire proximal colon and in the proximity of ulcerations in the distal colon. During DSS treatment the number of apoptotic cells in the epithelium of both colonic segments was increased. In the entire colon surface enterocytes became flattened and CA I negative during active disease (day 5-7). Additionally, CA I levels in the distal colon significantly decreased during this phase. In contrast, during the regenerative phase (day 28) CA I levels were restored in the distal colon and up-regulated in the proximal colon. During all disease phases increased numbers of goblet cells were observed in the surface epithelium of the entire colon. In the distal colon TFF3 expression extended to the bottom of the crypts during active disease. Finally, MUC2 and TFF3 expression was increased in the proximal colon during disease. CONCLUSION: DSS affected the epithelium by inhibiting proliferation and inducing apoptosis. DSS-induced inhibition of CA I expression indicates down-regulation of specific enterocyte functions. Accumulation of goblet cells in the surface epithelium and up-regulation of MUC2 and TFF3 expression in the proximal colon underline the importance of goblet cells in epithelial protection and repair, respectively.
|
Authors | Ingrid B Renes, Melissa Verburg, Daniëlle J P M Van Nispen, Jan A J M Taminiau, Hans A Büller, Jan Dekker, Alexandra W C Einerhand |
Journal | International journal of colorectal disease
(Int J Colorectal Dis)
Vol. 17
Issue 5
Pg. 317-26
(Sep 2002)
ISSN: 0179-1958 [Print] Germany |
PMID | 12172925
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antiviral Agents
- Biomarkers
- Growth Substances
- Muc2 protein, rat
- Mucin-2
- Mucins
- Muscle Proteins
- Neuropeptides
- Peptides
- TFF3 protein, rat
- Trefoil Factor-2
- Trefoil Factor-3
- Dextran Sulfate
- Carbonic Anhydrase I
- Bromodeoxyuridine
|
Topics |
- Animals
- Antiviral Agents
(pharmacology)
- Biomarkers
(analysis)
- Bromodeoxyuridine
(pharmacology)
- Carbonic Anhydrase I
(drug effects, genetics)
- Cell Death
(drug effects, physiology)
- Cell Differentiation
(drug effects, physiology)
- Colitis
(metabolism, physiopathology)
- Colon
(enzymology, physiopathology)
- Dextran Sulfate
(pharmacology)
- Disease Models, Animal
- Enterocytes
- Epithelial Cells
(cytology, drug effects, physiology)
- Gene Expression Regulation
(drug effects, physiology)
- Growth Substances
(genetics)
- Immunohistochemistry
- Intestinal Mucosa
(enzymology, physiopathology)
- Male
- Mucin-2
- Mucins
(drug effects, genetics)
- Muscle Proteins
- Neuropeptides
- Peptides
(drug effects, genetics)
- Rats
- Rats, Wistar
- Time Factors
- Trefoil Factor-2
- Trefoil Factor-3
- Weight Loss
(drug effects)
|