Abstract |
Eight spotted fever group (SFG) rickettsiae isolated from ticks in Japan were classified by phylogenetic analysis based on the nucleotide sequences of both the citrate synthase-encoding gene (gltA) and 190-kDa antigen-encoding gene (rOmpA). In the phylogenetic tree of gltA, strains DT-1 and FLA-1 isolated from the Dermacentor taiwanensis and Haemaphysalis frava ticks, respectively, were placed as Rickettsia japonica, and strains IO-1, IO-2, IO-25, IM-1 and IP-2 from genus Ixodes ticks were placed as Rickettsia helvetica. Strain AT-1 isolated from the Amblyomma testudinarium belonged to the cluster including Rickettsia akari, Rickettsia australis and Rickettsia felis. In the phylogenetic tree of the rOmpA, strains DT-1 and FLA-1 were placed as R. japonica, and strain AT-1 belonged to the cluster including Rickettsia cooleyi and the symbiont of Ixodes scapularis. The rOmpA fragments of 5 Ixodes isolates could not be amplified by PCR. The present study showed that strains DT-1 and FLA-1 were genotypically identical to R. japonica, and 5 Ixodes isolates were associated with the R. helvetica. Based on previous genotypic and antigenic data, and the phylogenetic analysis presented here, strain AT-1 should be considered as a new species among SFG rickettsiae.
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Authors | Mitsuhiro Ishikura, Hiromi Fujita, Shuji Ando, Kumiko Matsuura, Mamoru Watanabe |
Journal | Microbiology and immunology
(Microbiol Immunol)
Vol. 46
Issue 4
Pg. 241-7
( 2002)
ISSN: 0385-5600 [Print] Australia |
PMID | 12061626
(Publication Type: Journal Article)
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Chemical References |
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- rOmpA, Rickettsia
- Citrate (si)-Synthase
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Topics |
- Amino Acid Sequence
- Animals
- Bacterial Outer Membrane Proteins
(genetics)
- Bacterial Proteins
(genetics)
- Citrate (si)-Synthase
(genetics)
- Dog Diseases
(microbiology)
- Dogs
- Japan
- Molecular Sequence Data
- Phylogeny
- Rickettsia
(classification, genetics, isolation & purification)
- Sequence Homology, Amino Acid
- Ticks
(classification, microbiology)
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