Abstract |
Ribosomal protein (Rp) L6 is also defined as Taxreb107 (Tax responsive element binding protein 107) for its binding activity to the long terminal repeats of human T cell leukemia virus (HTLV)-I. We cloned the genomic gene of mouse RpL6/ Taxreb107 and analyzed its exon/intron structures. The promoter of RpL6/ Taxreb107 contains recognition sites for multiple transcription factors including nuclear factor ( NF)-kappa B. Luciferase reporter assay showed that the RpL6/ Taxreb107 promoter has a constitutive activity in transfected cells, and the constitutive activity depends on the intact promoter. Expression of HTLV-I viral protein Tax mildly but reproducibly induced RpL6/ Taxreb107 mRNA and promoter activity. We provide evidence suggesting that induction of RpL6/ Taxreb107 by Tax is at least partially mediated by the NF-kappa B site in the promoter of RpL6/ Taxreb107. Taken together, Tax up-regulates RpL6/ Taxreb107 and this may provide a feedback mechanism to facilitate proliferation of HTLV-I-infected cells and production of viral particles.
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Authors | Jishu Wang, Xi Yang, Peng Zhou, Hua Han |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1576
Issue 1-2
Pg. 219-24
(Jun 07 2002)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 12031506
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Complementary
- DNA-Binding Proteins
- Gene Products, tax
- NF-kappa B
- Ribosomal Proteins
- ribosomal protein L6
- tax-responsive-element-binding protein 107, mouse
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Topics |
- Animals
- COS Cells
- Cloning, Molecular
- DNA, Complementary
(biosynthesis, chemistry)
- DNA-Binding Proteins
(chemistry, genetics, metabolism)
- Embryo, Mammalian
(metabolism)
- Gene Expression Regulation, Developmental
- Gene Products, tax
(metabolism)
- Mice
- NF-kappa B
(chemistry, metabolism)
- Promoter Regions, Genetic
- Ribosomal Proteins
(chemistry, genetics, metabolism)
- Transfection
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