Isolation and identification of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid from the urine of a patient with sialuria.

Abstract

N-Acetylneuraminic acid preparations from the urine of a patient with sialuria contain 1--2% of 2-deoxy-2,3-dehydro-N-acetylneuraminic acid. This new human sialic acid was isolated by ion-exchange and partition chromatography. The structure has been elucidated by mass spectrometry and confirmed by comparison with the synthetic compound. The properties of this unsaturated sialic acid in the orcinol/Fe3+/HCl and the periodic acid/thiobarbituric acid tests as well as in thin-layer and gas-liquid chromatography are described. It does not react with acylneuraminate pyruvatelyase. The origin of this new human sialic acid is discussed.

• Authors: J P Kamerling, J F Vliegenthart
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 56 Issue 1 Pg. 253-8 (Aug 01 1975) ISSN: 0014-2956 [Print] England
• PMID: 1175622 (Publication Type: Journal Article)

Chemical References

• Sialic Acids

Topics

• Carbohydrate Metabolism, Inborn Errors (urine)
• Child
• Chromatography, Gas
• Chromatography, Thin Layer
• Humans
• Intellectual Disability (urine)
• Male
• Mass Spectrometry
• Sialic Acids (urine)