Abstract | OBJECTIVE: METHODS: PDE3B mRNA and protein were quantified by an RNase protection assay and Western blotting respectively. Membrane-bound PDE activities were also measured. RESULTS: In adipose tissues of KKAy mice, PDE3B mRNA, protein and membrane-bound PDE activity were reduced to 47%, 57% and 51% respectively relative to those in C57BL/6J control mice. JTT-501 increased PDE3B mRNA, protein and membrane-bound PDE activity by 2.2-, 1.6- and 1.7-fold respectively over those of untreated KKAy mice. In the liver, PDE3B gene expression remained unchanged in KKAy mice, and was not affected by JTT-501. JTT-501 reduced the elevated levels of serum insulin, glucose, FFA and triglyceride in KKAy mice. CONCLUSIONS: PDE3B gene expression was specifically reduced in the adipose tissues of KKAy mice. JTT-501 restored this reduced gene expression with an accompanying improvement in elevated serum FFA and insulin resistance.
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Authors | Y Tang, H Osawa, H Onuma, M Hasegawa, T Nishimiya, M Ochi, H Makino |
Journal | European journal of endocrinology
(Eur J Endocrinol)
Vol. 145
Issue 1
Pg. 93-9
(Jul 2001)
ISSN: 0804-4643 [Print] England |
PMID | 11415857
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Fatty Acids
- Hypoglycemic Agents
- Insulin
- Isoxazoles
- RNA, Messenger
- Triglycerides
- 3',5'-Cyclic-AMP Phosphodiesterases
- Cyclic Nucleotide Phosphodiesterases, Type 3
- Pde3b protein, mouse
- JTT 501
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Topics |
- 3',5'-Cyclic-AMP Phosphodiesterases
(analysis, biosynthesis, genetics)
- Adipocytes
(drug effects, enzymology)
- Adipose Tissue
(drug effects, enzymology)
- Animals
- Blood Glucose
(metabolism)
- Blotting, Western
- Cyclic Nucleotide Phosphodiesterases, Type 3
- Diabetes Mellitus
(enzymology)
- Diabetes Mellitus, Experimental
(enzymology)
- Epididymis
(drug effects, enzymology)
- Fatty Acids
(blood)
- Female
- Gene Expression Regulation, Enzymologic
(drug effects)
- Hypoglycemic Agents
(pharmacology)
- Insulin
(blood)
- Insulin Resistance
(physiology)
- Isoxazoles
(pharmacology)
- Liver
(drug effects, enzymology)
- Male
- Mice
- Mice, Inbred C57BL
- Obesity
- RNA, Messenger
(genetics, metabolism)
- Triglycerides
(blood)
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