Determination of parthenolide in selected feverfew products by liquid chromatography.

Abstract

The migraine prophylactic herb feverfew (Tanacetum parthenium L.) is marketed in the United States in a variety of forms and compositions. Although its therapeutic efficacy is still uncertain, the sesquiterpene lactone parthenolide is the constituent recommended to be measured for quality control of feverfew preparations. A validated liquid chromatographic method was developed and used to estimate parthenolide in a number of U.S. feverfew market products formulated as capsules, tablets, or crude powder. The method uses a Lichrosphere 5 C18 column, a mobile phase consisting of 50 mM NaH2PO4 in H2O (solvent A), and CH3CN-MeOH (90 + 10, v/v; solvent B). Elution was run at a flow rate of 1.0 mL/min with a linear gradient of 50-15% A in B over 20 min and UV detection at 210 nm. The correlation coefficient for the calibration curve was 0.9999 over the range of 0.00-0.400 mg/mL. Overall recovery of parthenolide was 103.1%.

• Authors: E A Abourashed, I A Khan, E A Abourashed, I A Khan
• Journal: Journal of AOAC International (J AOAC Int) 2000 Jul-Aug Vol. 83 Issue 4 Pg. 789-92 ISSN: 1060-3271 [Print] England
• PMID: 10995104 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Pharmaceutical Preparations
• Sesquiterpenes
• Solutions
• parthenolide

Topics

• Anti-Inflammatory Agents, Non-Steroidal (analysis)
• Chromatography, Liquid (methods)
• Drug Costs
• Drug Labeling
• Humans
• Linear Models
• Magnoliopsida (chemistry)
• Migraine Disorders (drug therapy)
• Pharmaceutical Preparations (analysis)
• Plants, Medicinal (chemistry)
• Quality Control
• Sensitivity and Specificity
• Sesquiterpenes (analysis)
• Solutions
• Spectrophotometry, Ultraviolet