Identification of coronary thrombus with a IIb/IIIa platelet inhibitor radiopharmaceutical, technetium-99m DMP-444: A canine model.

Abstract

BACKGROUND:

A diagnostic test that could distinguish between stable and unstable coronary atherosclerotic plaques would be useful. We tested the ability of a new glycoprotein IIb/IIIa platelet inhibitor DMP-444, labeled with technetium (Tc)-99 m, to identify platelet-rich thrombus by nuclear imaging in a canine model.

METHODS AND RESULTS:

Combinations of a flow-limiting stenosis and 0 to 15 minutes of endothelial electrical stimulation at a site in the left anterior descending coronary artery were used to induce varying amounts of thrombus formation. In 10 animals with markedly positive nuclear images after the injection of Tc-99m DMP-444, the presence of platelet-rich thrombus was confirmed postmortem by gross appearance, high nuclear counts, and abundant platelets on electron microscopy. The 10 animals with negative images had lower counts, smaller thrombus weights (P<0.05 for each), and fewer platelets by electron microscopy.

CONCLUSIONS:

Activated platelets participating in acute thrombus formation can be accurately detected by nuclear imaging using Tc-99 m DMP-444.

• Authors: J Mitchel, D Waters, T Lai, M White, T Alberghini, A Salloum, D Knibbs, D Li, G V Heller
• Journal: Circulation (Circulation) Vol. 101 Issue 14 Pg. 1643-6 (Apr 11 2000) ISSN: 1524-4539 [Electronic] United States
• PMID: 10758044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Organotechnetium Compounds
• Platelet Glycoprotein GPIIb-IIIa Complex
• Radiopharmaceuticals
• Sulfhydryl Compounds
• technetium Tc 99m 2,3-dimercaptopropionic acid

Topics

• Animals
• Blood Platelets (pathology)
• Coronary Thrombosis (blood, diagnosis, pathology)
• Dogs
• Feasibility Studies
• Microscopy, Electron
• Organotechnetium Compounds
• Platelet Activation
• Platelet Count
• Platelet Glycoprotein GPIIb-IIIa Complex (antagonists & inhibitors)
• Radiopharmaceuticals
• Sulfhydryl Compounds