Selectin on activated platelets enhances neutrophil endothelial adherence in myocardial reperfusion injury.

Abstract

OBJECTIVES:

The glycoprotein P-selectin is an adhesion molecule that is rapidly expressed on the surface of platelets and endothelium during the inflammatory process. P-selectin on endothelium has been reported to play an important role in reperfusion injury. However, little is known regarding P-selectin on platelets in contributing to the pathophysiology of myocardial reperfusion injury. In this study, we hypothesized that P-selectin on platelets may enhance neutrophil endothelial adherence and this may play a role in neutrophil-mediated reperfusion injury.

METHODS:

Endothelial cells, cardiomyocytes, platelets and neutrophils were isolated from adult rats. Endothelial cells and cardiomyocytes were cultivated in a co-culture system. After exposure to hypoxia and reoxygenation, neutrophil adherence and migration were examined.

RESULTS:

After exposure to 6 h of hypoxia, endothelial cells co-incubated with platelets showed significantly greater neutrophil adherence (63.1 +/- 4.0%) and migration (78.2 +/- 6.7%) than endothelial cells alone (adhesion: 44.2 +/- 2.8%, migration: 57.9 +/- 4.9%). These increases were significantly inhibited (adhesion: 42.1 +/- 3.5%, migration: 65.5 +/- 3.8%) by an anti-P-selectin monoclonal antibody. Moreover, the superoxide-anion production was significantly elevated when activated platelets were added to neutrophils. This enhanced production was also inhibited by anti-P-selectin antibody.

CONCLUSION:

The presence of activated platelets enhanced neutrophil adhesion and migration process after hypoxia reoxygenation. This process may occur following platelet-neutrophil interactions via P-selectin and subsequent neutrophil activation.

• Authors: S Kogaki, Y Sawa, T Sano, T Matsushita, T Ohata, S Kurotobi, S J Tojo, H Matsuda, S Okada
• Journal: Cardiovascular research (Cardiovasc Res) Vol. 43 Issue 4 Pg. 968-73 (Sep 1999) ISSN: 0008-6363 [Print] England
• PMID: 10615424 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• P-Selectin

Topics

• Animals
• Antibodies, Monoclonal (pharmacology)
• Blood Platelets (physiology)
• Cell Adhesion
• Cell Movement
• Cells, Cultured
• Coculture Techniques
• Endothelium, Vascular (physiology)
• Male
• Mice
• Myocardial Reperfusion Injury (pathology, physiopathology)
• Myocardium (pathology)
• Neutrophils (physiology)
• P-Selectin (immunology, physiology)
• Platelet Activation
• Rats
• Rats, Sprague-Dawley