Functional electrical stimulation exercise increases GLUT-1 and GLUT-4 in paralyzed skeletal muscle.

Abstract

The study purpose was to determine the effect of functional electrical stimulation (FES)-leg cycle ergometer training (30 minutes on 3 d/wk for 8 weeks) on the GLUT-1 and GLUT-4 content of paralyzed skeletal muscle. Biopsy samples of vastus lateralis muscle were obtained pre- and post-training from five individuals with motor-complete spinal cord injury ([SCI] four men and one woman aged 31 to 50 years, 3 to 25 years postinjury involving C5-T8). Western blot analysis indicated that GLUT-1 increased by 52% and GLUT-4 increased by 72% with training (P < .05). This coincided with an increase in the muscle oxidative capacity as indicated by a 56% increase in citrate synthase (CS) activity (P < .05) and an improvement in the insulin sensitivity index as determined from oral glucose tolerance tests (P < .05). It is concluded that FES endurance training is effective to increase glucose transporter protein levels in paralyzed skeletal muscle of individuals with SCI.

• Authors: P D Chilibeck, G Bell, J Jeon, C B Weiss, G Murdoch, I MacLean, E Ryan, R Burnham
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 48 Issue 11 Pg. 1409-13 (Nov 1999) ISSN: 0026-0495 [Print] United States
• PMID: 10582549 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glucose Transporter Type 1
• Glucose Transporter Type 4
• Monosaccharide Transport Proteins
• Muscle Proteins
• SLC2A1 protein, human
• SLC2A4 protein, human
• Citrate (si)-Synthase

Topics

• Adult
• Biopsy
• Blotting, Western
• Citrate (si)-Synthase (metabolism)
• Electric Stimulation Therapy
• Exercise Therapy
• Female
• Glucose Tolerance Test
• Glucose Transporter Type 1
• Glucose Transporter Type 4
• Humans
• Male
• Middle Aged
• Monosaccharide Transport Proteins (metabolism)
• Muscle Proteins
• Muscle, Skeletal (enzymology, metabolism)
• Oxidation-Reduction
• Paralysis (enzymology, metabolism)