Increased activity of membrane glycoprotein PC-1 in the fibroblasts from non-insulin-dependent diabetes mellitus patients with insulin resistance.

Abstract

Although most of patients with non-insulin-dependent diabetes mellitus (NIDDM) have insulin resistance, it is unknown whether a molecule might interfere with insulin action. Membrane glycoprotein PC-1 (plasma cell antigen-1), which inhibits insulin receptor tyrosine kinase activity, was isolated from fibroblasts of NIDDM patients. Because PC-1 content in skeletal muscle and adipose tissue correlated with whole body insulin sensitivity, PC-1 might play a role in insulin resistance. In order to know whether PC-1 activity of fibroblasts is also elevated in Japanese NIDDM patients, and whether PC-1 activity correlates with the parameters of insulin resistance in vivo or not, we measured PC-1 activity of cultured fibroblasts from 17 patients with NIDDM and seven healthy controls. PC-1 activity of the NIDDM patients was 85.2 +/- 33.1 nmol/mg per min (mean +/- S.D.), and was higher than that of healthy controls (42.6 +/- 12.7 nmol/mg per min, P = 0.0002). Insulin sensitivity was measured in 11 of 17 NIDDM patients by the artificial pancreas. PC-1 activity of the patients with insulin resistance (glucose infusion rate < 3.0 mg/kg per min, n = 7) was elevated to 99.9 +/- 31.9 nmol/mg per min, while that of the other patients (n = 4) was 55.3 +/- 7.5 nmol/mg per min (P = 0.003). In conclusion, glycoprotein PC-1 activity of dermal fibroblasts is correlated with insulin resistance in patients with NIDDM.

• Authors: S Teno, H Kanno, S Oga, S Kumakura, R Kanamuro, Y Iwamoto
• Journal: Diabetes research and clinical practice (Diabetes Res Clin Pract) Vol. 45 Issue 1 Pg. 25-30 (Aug 1999) ISSN: 0168-8227 [Print] Ireland
• PMID: 10499882 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Glycated Hemoglobin A
• Membrane Glycoproteins
• Phosphoric Diester Hydrolases
• ectonucleotide pyrophosphatase phosphodiesterase 1
• Pyrophosphatases

Topics

• Adult
• Aged
• Biopsy
• Blood Glucose (analysis)
• Cells, Cultured
• Diabetes Mellitus, Type 2 (physiopathology)
• Female
• Fibroblasts (chemistry)
• Glucose Clamp Technique
• Glycated Hemoglobin (analysis)
• Humans
• Insulin Resistance (physiology)
• Male
• Membrane Glycoproteins (analysis, physiology)
• Middle Aged
• Phosphoric Diester Hydrolases
• Pyrophosphatases
• Scintillation Counting
• Skin (pathology)