Fertilin beta peptides inhibit sperm binding to zona-free eggs in a homologous baboon in vitro fertilization system.

Abstract

This study developed a baboon in vitro fertilization system that can be used in testing defined gamete antigens for fertility effects. A laparoscopic procedure that proved very valuable in retrieving eggs from female baboons for in vitro studies was developed. On average, 30 +/- 5 (SD) eggs were harvested per female baboon per cycle. Micromolar quantities of hexapeptides or a 28 aa residue peptide, all corresponding to fertilin beta disintegrin domain, competitively inhibited the binding of zona-free baboon eggs by baboon sperm in vitro. This study demonstrated that fertilin beta has a required role in baboon fertilization.

• Authors: P G Mwethera, A Makokha, D Chai
• Journal: Contraception (Contraception) Vol. 59 Issue 2 Pg. 131-5 (Feb 1999) ISSN: 0010-7824 [Print] United States
• PMID: 10361628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Membrane Glycoproteins
• Peptide Fragments
• ADAM Proteins
• Fertilins
• Metalloendopeptidases

Topics

• ADAM Proteins
• Amino Acid Sequence
• Animals
• Female
• Fertilins
• Fertilization in Vitro (methods, veterinary)
• Male
• Membrane Glycoproteins (chemistry, pharmacology, physiology)
• Metalloendopeptidases (chemistry, pharmacology, physiology)
• Molecular Sequence Data
• Ovum (drug effects, physiology)
• Papio
• Peptide Fragments (chemistry, pharmacology)
• Sperm Motility
• Sperm-Ovum Interactions (drug effects, physiology)
• Spermatozoa (drug effects, physiology)
• Zona Pellucida (physiology)