Differential expression of beta1,3galactosyltransferases in human colon cells derived from adenocarcinomas or normal mucosa.

Abstract

Two beta1,3galactosyltransferases are detected in human colon cells: one corresponds to beta3GalT1, the other (beta3GalTx) is found to be different from any cloned beta3GalT since in vitro it utilizes GlcNAc very efficiently under specific reaction conditions. Expression of beta3GalT1 transcript is high in normal colon mucosa and control neuroectodermal cells, which do not express sialyl-Lewis a antigen, and low in colon adenocarcinoma cells, as assessed by competitive RT-PCR. beta3GalTx activity is high in adenocarcinoma cells expressing sialyl-Lewis a and undetectable in all other cells, suggesting differential involvement and opposite regulation of such enzymes during carcinogenesis.

• Authors: A Bardoni, M Valli, M Trinchera
• Journal: FEBS letters (FEBS Lett) Vol. 451 Issue 1 Pg. 75-80 (May 14 1999) ISSN: 0014-5793 [Print] England
• PMID: 10356986 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CA-19-9 Antigen
• DNA, Complementary
• Gangliosides
• sialyl Le(a) ganglioside
• Galactosyltransferases

Topics

• Adenocarcinoma (enzymology, genetics)
• Animals
• Base Sequence
• CA-19-9 Antigen
• CHO Cells
• Colonic Neoplasms (enzymology, genetics)
• Cricetinae
• DNA, Complementary
• Galactosyltransferases (genetics, metabolism)
• Gangliosides (biosynthesis)
• Gastric Mucosa (enzymology)
• Gene Expression Regulation, Enzymologic
• HT29 Cells
• Humans
• Molecular Sequence Data
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured