Amelioration by glucose-6-phosphate and NADP of potato glycoalkaloid inhibition in cell, enzyme and liposome assays.

Abstract

Lysis of human erythrocytes by 20 microM chaconine was reduced by 0.5 mM glucose-6-phosphate (G6P) and NADP. Both compounds caused approximately 50% inhibition of haemolysis at 1 mM. Glucose, glucose-1-phosphate, rhamnose, galactose and galactose-6-phosphate were ineffective; NAD was effective, although not to the extent of NADP. Of the tested sugars, only G6P reduced solanine-induced haemolysis. G6P also reduced the synergistic haemolytic action of solanine and chaconine in combination. G6P and NADP at or above 5 mM antagonised chaconine-induced betanin loss from excised red beet root discs; NADP was more effective than G6P. Disruption of PC/cholesterol liposomes by chaconine and inhibition of acetylcholinesterase by chaconine or solanine, were unaffected by up to 10 mM NADP or 50 mM G6P.

• Authors: J G Roddick, A L Leonard
• Journal: Phytochemistry (Phytochemistry) Vol. 51 Issue 1 Pg. 23-7 (May 1999) ISSN: 0031-9422 [Print] England
• PMID: 10349728 (Publication Type: Journal Article)

Chemical References

• Cholinesterase Inhibitors
• Liposomes
• Solanine
• alpha-chaconine
• NADP
• Glucose-6-Phosphate
• Acetylcholinesterase

Topics

• Acetylcholinesterase (drug effects, metabolism)
• Cholinesterase Inhibitors (pharmacology)
• Glucose-6-Phosphate (pharmacology)
• Hemolysis (drug effects)
• Humans
• Liposomes
• NADP (pharmacology)
• Solanine (analogs & derivatives, antagonists & inhibitors, pharmacology)
• Solanum tuberosum (chemistry)