Macrophage inflammatory protein 1 alpha expression by synovial fluid neutrophils in rheumatoid arthritis.

Abstract

OBJECTIVE:

To determine the contribution made by synovial fluid (SF) neutrophils to the augmented expression of macrophage inflammatory protein 1 alpha (MIP-1alpha) in rheumatoid arthritis (RA).

METHODS:

Neutrophils were isolated from samples of SF from RA patients and peripheral blood (PB) samples from RA patients and healthy controls. Cell associated MIP-1alpha was visualised immunohistochemically, and cell associated MIP-1alpha as well as MIP-1alpha secreted into the SF was assayed by ELISA. Steady state expression of MIP-1alpha mRNA was assessed by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS:

Freshly isolated SF neutrophils contained significantly higher concentrations of both MIP-1alpha protein and its transcript than PB neutrophils from either RA patients or healthy controls; incubation in the absence or presence of tumour necrosis factor alpha for 24 hours resulted in a significant increase in MIP-1alpha secretion by RA SF neutrophils compared with neutrophils obtained from either normal PB or RA PB; and expression of MIP-1alpha by SF neutrophils was well correlated with both RA disease activity and SF mononuclear cell (MNC) counts.

CONCLUSION:

Expression and secretion of MIP-1alpha by SF neutrophils may be indicative of local and systemic inflammation in RA. Moreover, this C-C chemokine may contribute to the recruitment of MNCs from the bloodstream into synovial joints and tissues.

• Authors: Y Hatano, T Kasama, H Iwabuchi, R Hanaoka, H T Takeuchi, L Jing, Y Mori, K Kobayashi, M Negishi, H Ide, M Adachi
• Journal: Annals of the rheumatic diseases (Ann Rheum Dis) Vol. 58 Issue 5 Pg. 297-302 (May 1999) ISSN: 0003-4967 [Print] England
• PMID: 10225815 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Chemokine CCL3
• Chemokine CCL4
• Macrophage Inflammatory Proteins
• RNA, Messenger

Topics

• Adult
• Aged
• Arthritis, Rheumatoid (immunology)
• Biomarkers (analysis)
• Chemokine CCL3
• Chemokine CCL4
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunohistochemistry
• Macrophage Inflammatory Proteins (analysis, genetics)
• Male
• Middle Aged
• Neutrophils (chemistry)
• RNA, Messenger (analysis)
• Reverse Transcriptase Polymerase Chain Reaction
• Statistics, Nonparametric
• Synovial Fluid (immunology)