Abstract | OBJECTIVE: To evaluate the therapeutic efficacy of nonmitogenic anti-CD3 monoclonal antibody (MAb) in a preexisting autoaggressive response, using the MRL-lpr/lpr (MRL/l) murine model of autoimmune disease. METHODS: Female MRL/l mice, 8-10 weeks of age, were treated with nonmitogenic anti-CD3 MAb or phosphate buffered saline and effects on mortality, lymphadenopathy, T cell phenotypes, anti- DNA titers, and total IgG titers were measured. RESULTS: Nonmitogenic anti-CD3 MAb treatment resulted in a dramatic reduction in lymphadenopathy and mortality, as well as an early reduction in alpha/beta+, CD4-, CD8-, Thy+, B220+ (double-negative) lymph node cells. No significant effects on anti- DNA or IgG titers were observed. No morbidity was observed following administration of nonmitogenic anti-CD3 MAb. CONCLUSION: A short course of treatment with nonmitogenic anti-CD3 MAb can suppress preexisting autoimmune responses without inducing the cytokine-mediated toxicity characteristic of mitogenic forms of anti-CD3 MAb. The use of nonmitogenic anti-CD3 MAb may be efficacious in the clinical setting for the treatment of T cell-mediated autoimmune disorders.
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Authors | M Henrickson, E H Giannini, R Hirsch |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 37
Issue 4
Pg. 587-94
(Apr 1994)
ISSN: 0004-3591 [Print] United States |
PMID | 8147938
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Autoimmune Diseases
(mortality, prevention & control)
- Disease Models, Animal
- Female
- Lymphatic Diseases
(pathology, prevention & control)
- Lymphocyte Depletion
- Mice
- Muromonab-CD3
(administration & dosage)
- Survival Rate
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